Fig. 3. PKM2 knockdown decreased the colony formation ability of prostate cancer cells. (A) Representative photographs of the colony formation assay showing the proliferation of DU145 cells transfected with the PKM2 siRNAs in six-well plates. The cell colonies were scored visually and were counted using a light microscope. (B) Quantitative analysis of the colony number. Data are representative of three independent experiments. One-way ANOVA was used to compare the means of different groups. Differences between the means were considered to be significant at P<0.05 by using Tukey multiple comparison tests; ^*P<0.05, **P<0.01, and ***P<0.001 compared with the normal control cells.